2,43
1,65
1,98
0,73
1,88
1,81
2,43
2.2 Standardne snovi, uporabljene v umeritveni krivulji porazdelitve relativne molekulske mase: inzulin, mikopeptidi, glicin-glicin-tirozin-arginin, glicin-glicin-glicin
3 Instrumenti in oprema
23.2
21,4
22.2
16.1
22,3
20,8
23,9
27,5
Na splošno je delež aminokislin v izdelkih Sustar višji kot v izdelkih Zinpro.
8. del Učinki uporabe
Vpliv različnih virov elementov v sledovih na proizvodno uspešnost in kakovost jajc kokoši nesnic v poznem obdobju nesnosti
Proizvodni proces
Ciljno usmerjena tehnologija kelacije
Tehnologija strižne emulgacije
Tehnologija tlačnega pršenja in sušenja
Tehnologija hlajenja in razvlaževanja
Napredna tehnologija za nadzor okolja
Dodatek A: Metode za določanje relativne porazdelitve molekulske mase peptidov
Sprejetje standarda: GB/T 22492-2008
1 Načelo testiranja:
Določena je bila z visokozmogljivo gelsko filtracijsko kromatografijo. To pomeni, da so kot stacionarno fazo uporabili porozno polnilo in na podlagi razlike v relativni molekulski masi komponent vzorca za ločevanje, zaznane pri peptidni vezi ultravijolične absorpcijske valovne dolžine 220 nm, z uporabo namenske programske opreme za obdelavo podatkov za določanje porazdelitve relativne molekulske mase z gelsko filtracijsko kromatografijo (tj. programske opreme GPC) obdelali kromatograme in njihove podatke ter izračunali velikost relativne molekulske mase sojinega peptida in območje porazdelitve.
2. Reagenti
Eksperimentalna voda mora ustrezati specifikacijam sekundarne vode v GB/T6682, uporabljeni reagenti, razen za posebne določbe, pa morajo biti analitično čisti.
2.1 Reagenti vključujejo acetonitril (kromatografsko čist), trifluorocetno kislino (kromatografsko čista),
2.2 Standardne snovi, uporabljene v umeritveni krivulji porazdelitve relativne molekulske mase: inzulin, mikopeptidi, glicin-glicin-tirozin-arginin, glicin-glicin-glicin
3 Instrumenti in oprema
3.1 Visokozmogljiv tekočinski kromatograf (HPLC): kromatografska delovna postaja ali integrator z UV-detektorjem in programsko opremo za obdelavo podatkov GPC.
3.2 Enota za vakuumsko filtracijo in odplinjevanje mobilne faze.
3.3 Elektronska tehtnica: graduirana vrednost 0,000 1 g.
4 koraki delovanja
4.1 Kromatografski pogoji in poskusi prilagoditve sistema (referenčni pogoji)
- 4.1.1 Kromatografska kolona: TSKgelG2000swxl300 mm × 7,8 mm (notranji premer) ali druge gelske kolone iste vrste s podobnim delovanjem, primerne za določanje beljakovin in peptidov.
- 4.1.2 Mobilna faza: acetonitril + voda + trifluoroocetna kislina = 20 + 80 + 0,1.
- 4.1.3 Valovna dolžina detekcije: 220 nm.
- 4.1.4 Pretok: 0,5 ml/min.
- 4.1.5 Čas zaznavanja: 30 min.
- 4.1.6 Prostornina vbrizganega vzorca: 20 μL.
- 4.1.7 Temperatura kolone: sobna temperatura.
- 4.1.8 Da bi kromatografski sistem izpolnjeval zahteve glede detekcije, je bilo določeno, da pri zgoraj navedenih kromatografskih pogojih učinkovitost kolone gelske kromatografije, tj. teoretično število plošč (N), ni manjša od 10000, izračunano na podlagi vrhov tripeptidnega standarda (glicin-glicin-glicin).
- 4.2 Izdelava standardnih krivulj relativne molekulske mase
- Zgornje različne raztopine peptidnih standardov z relativno molekulsko maso in masno koncentracijo 1 mg/ml so bile pripravljene z ujemanjem mobilne faze, zmešane v določenem razmerju in nato filtrirane skozi organsko fazno membrano z velikostjo por 0,2 μm~0,5 μm ter vbrizgane v vzorec, nato pa so bili pridobljeni kromatogrami standardov. Kalibracijske krivulje relativne molekulske mase in njihove enačbe so bile pridobljene z grafom logaritma relativne molekulske mase glede na retencijski čas ali z linearno regresijo.
4.3 Obdelava vzorca
V 10 ml merilno bučko natančno odtehtajte 10 mg vzorca, dodajte malo mobilne faze in ultrazvočno stresajte 10 minut, da se vzorec popolnoma raztopi in premeša. Razredčite z mobilno fazo do tehtnice in nato filtrirajte skozi membrano iz organske faze z velikostjo por 0,2 μm ~ 0,5 μm. Filtrat pa analizirajte v skladu s kromatografskimi pogoji v A.4.1.
- 5. Izračun relativne porazdelitve molekulskih mas
- Po analizi raztopine vzorca, pripravljene v 4.3, pod kromatografskimi pogoji iz 4.1, lahko relativno molekulsko maso vzorca in njegovo porazdelitveno območje dobimo tako, da kromatografske podatke vzorca vnesemo v umeritveno krivuljo 4.2 s programsko opremo za obdelavo podatkov GPC. Porazdelitev relativnih molekulskih mas različnih peptidov lahko izračunamo z metodo normalizacije površine vrhov po formuli: X=A/A skupaj×100
- V formuli: X - masni delež peptida z relativno molekulsko maso v celotnem peptidu v vzorcu, %;
- A - Površina vrha peptida z relativno molekulsko maso;
- Skupaj A - vsota površin vrhov vsakega peptida z relativno molekulsko maso, izračunana na eno decimalno mesto natančno.
- 6 Ponovljivost
- Absolutna razlika med dvema neodvisnima določitvama, dobljenima v pogojih ponovljivosti, ne sme presegati 15 % aritmetične sredine obeh določitev.
- Dodatek B: Metode za določanje prostih aminokislin
- Sprejetje standarda: Q/320205 KAVN05-2016
- 1.2 Reagenti in materiali
- Ledocetna kislina: analitično čista
- Perklorna kislina: 0,0500 mol/L
- Indikator: 0,1 % kristalno vijoličen indikator (ledena ocetna kislina)
- 2. Določanje prostih aminokislin
Vzorce smo sušili pri 80 °C 1 uro.
Vzorec postavite v suho posodo, da se naravno ohladi na sobno temperaturo ali na uporabno temperaturo.V 250 ml suho erlenmajerico odtehtajte približno 0,1 g vzorca (natančno na 0,001 g).Hitro nadaljujte z naslednjim korakom, da preprečite vpijanje vlage iz okolja v vzorec.Dodajte 25 ml ledocetne kisline in dobro mešajte največ 5 minut.Dodajte 2 kapljici indikatorja kristal vijoličnegaTitriramo s standardno titracijsko raztopino perklorne kisline s koncentracijo 0,0500 mol/L (±0,001), dokler se raztopina ne obarva iz vijolične v končno točko.
Zapišite količino porabljene standardne raztopine.
- Hkrati izvedite slepi test.
- 3. Izračun in rezultati
- Vsebnost prostih aminokislin X v reagentu je izražena kot masni delež (%) in se izračuna po formuli: X = C × (V1-V0) × 0,1445/M × 100 %, po formuli:
- C - Koncentracija standardne raztopine perklorne kisline v molih na liter (mol/L)
- V1 - Prostornina, uporabljena za titracijo vzorcev s standardno raztopino perklorove kisline, v mililitrih (mL).
- Vo - volumen, uporabljen za titracijo s standardno raztopino perklorove kisline, v mililitrih (mL);
M - Masa vzorca v gramih (g).
| 0,1445: Povprečna masa aminokislin, ki ustreza 1,00 mL standardne raztopine perklorove kisline [c (HClO4) = 1,000 mol / L]. | 4.2.3 Standardna titracijska raztopina cerijevega sulfata: koncentracija c [Ce(SO4)2] = 0,1 mol/L, pripravljena v skladu z GB/T601. | |
| Sprejetje standardov: Q/70920556 71-2024 | 1. Načelo določanja (Fe kot primer) | Železovi kompleksi aminokislin imajo zelo nizko topnost v brezvodnem etanolu, prosti kovinski ioni pa so topni v brezvodnem etanolu, zato je bila razlika v topnosti med obema v brezvodnem etanolu uporabljena za določitev stopnje kelacije železovih kompleksov aminokislin. |
| V formuli: V1 - volumen standardne raztopine cerijevega sulfata, porabljene za titracijo preskusne raztopine, ml; | Brezvodni etanol; ostalo je enako kot v točki 4.5.2 v GB/T 27983-2011. | 3. Koraki analize |
| Vzporedno izvedite dva poskusa. Odtehtajte 0,1 g vzorca, sušenega pri 103 ± 2 ℃ 1 uro, natančno na 0,0001 g, dodajte 100 ml brezvodnega etanola, da se raztopi, filtrirajte, ostanek filtra vsaj trikrat sperite s 100 ml brezvodnega etanola, nato ostanek prenesite v 250 ml erlenmajerico, dodajte 10 ml raztopine žveplove kisline v skladu s točko 4.5.3 v GB/T27983-2011 in nato izvedite naslednje korake v skladu s točko 4.5.3 »Segrejte, da se raztopi, in nato pustite, da se ohladi« v GB/T27983-2011. Hkrati izvedite slepi preskus. | 4. Določanje skupne vsebnosti železa | 4.1 Načelo določitve je enako kot v točki 4.4.1 v GB/T 21996-2008. |
4.2. Reagenti in raztopine
| 4.2.1 Mešana kislina: V 700 ml vode dodajte 150 ml žveplove kisline in 150 ml fosforne kisline ter dobro premešajte. | 4.2.2 Indikatorska raztopina natrijevega difenilamin sulfonata: 5 g/L, pripravljena v skladu z GB/T603. | 4.2.3 Standardna titracijska raztopina cerijevega sulfata: koncentracija c [Ce(SO4)2] = 0,1 mol/L, pripravljena v skladu z GB/T601. | |
| 4.3 Koraki analize | Vzporedno izvedite dva poskusa. Odtehtajte 0,1 g vzorca na 0,20001 g natančno, ga dajte v 250 ml erlenmajerico, dodajte 10 ml mešane kisline, po raztopitvi dodajte 30 ml vode in 4 kapljice indikatorske raztopine natrijevega dianilin sulfonata ter nato izvedite naslednje korake v skladu s točko 4.4.2 v GB/T21996-2008. Hkrati izvedite slepi preskus. | 4.4 Predstavitev rezultatov | Skupna vsebnost železa X1 v aminokislinskih železovih kompleksih, izražena v masnem deležu železa, je bila izračunana po formuli (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - standardna raztopina cerijevega sulfata, porabljena za titracijo slepe raztopine, ml; | V0 - standardna raztopina cerijevega sulfata, porabljena za titracijo slepe raztopine, ml; | C - Dejanska koncentracija standardne raztopine cerijevega sulfata, mol/L5. Izračun vsebnosti železa v kelatihVsebnost železa X2 v kelatu, izražena kot masni delež železa, vrednost, izražena v %, je bila izračunana po formuli: x2 = ((V1-V2) × C × 0,05585)/m1 × 100 |
| V formuli: V1 - volumen standardne raztopine cerijevega sulfata, porabljene za titracijo preskusne raztopine, ml; | V2 - standardna raztopina cerijevega sulfata, porabljena za titracijo slepe raztopine, ml;nom1 - masa vzorca, g. Kot rezultat določitve se vzame aritmetična sredina rezultatov vzporednih določitev, absolutna razlika rezultatov vzporednih določitev pa ne sme presegati 0,3 %. | 0,05585 - masa železovega oksida, izražena v gramih, kar ustreza 1,00 ml standardne raztopine cerijevega sulfata C[Ce(SO4)2.4H20] = 1,000 mol/L.nom1 - masa vzorca, g. Kot rezultat določitve se vzame aritmetična sredina rezultatov vzporednih določitev, absolutna razlika rezultatov vzporednih določitev pa ne sme presegati 0,3 %. | 6. Izračun stopnje kelacijeStopnja kelacije X3, vrednost izražena v %, X3 = X2/X1 × 100Dodatek C: Metode za določanje stopnje kelacije zdravila Zinpro |
Sprejetje standarda: Q/320205 KAVNO7-2016
1. Reagenti in materiali
a) Ledocetna kislina: analitsko čista; b) Perklorova kislina: 0,0500 mol/L; c) Indikator: 0,1 % kristalno vijolični indikator (ledenocetna kislina)
2. Določanje prostih aminokislin
2.1 Vzorce smo sušili pri 80 °C 1 uro.
2.2 Vzorec postavite v suho posodo, da se naravno ohladi na sobno temperaturo ali na uporabno temperaturo.
2.3 V 250 ml suho erlenmajerico odtehtajte približno 0,1 g vzorca (natančno na 0,001 g).
2.4 Hitro nadaljujte z naslednjim korakom, da preprečite vpijanje vlage iz okolja v vzorec.
2.5 Dodajte 25 ml ledocetne kisline in dobro mešajte največ 5 minut.
2.6 Dodajte 2 kapljici indikatorja kristal vijoličnega.
2.7 Titrirajte s standardno titracijsko raztopino perklorove kisline s koncentracijo 0,0500 mol/L (±0,001), dokler se raztopina ne spremeni iz vijolične v zeleno, 15 sekund, ne da bi se pri tem na koncu raztopine spremenila barva.
2.8 Zapišite količino porabljene standardne raztopine.
2.9 Slepi preskus izvedite hkrati.
- 3. Izračun in rezultati
- Katalonski
- Physicochemical parameters
V1 - Prostornina, uporabljena za titracijo vzorcev s standardno raztopino perklorove kisline, v mililitrih (mL).
Vo - volumen, uporabljen za titracijo s standardno raztopino perklorove kisline, v mililitrih (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Naslov: št. 147 Qingpu Road, mesto Shouan, okrožje Pujiang, mesto Chengdu, provinca Sečuan, Kitajska
Telefon: 86-18880477902
Izdelki
Anorganski minerali v sledovih
- Organski elementi v sledovih
- svahili
- Prilagojena storitev
- Hitre povezave
Profil podjetja
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| gudžaratščina | Kliknite za povpraševanje | © Avtorske pravice - 2010–2025: Vse pravice pridržane. | Zemljevid spletnega mesta NAJBOLJŠE ISKANJE Telefon |
| Tel. | 86-18880477902 | Javanski | E-pošta |
| 8618880477902 | Kitajščina | Francoščina | |
| Bird | Kitajščina | Francoščina | Nemščina Španščina |
| Aquatic animals | Japonščina | Korejščina | arabščina Grščina |
| turščina | Italijanščina | ||
| Ruminant animal g/head day | January 0.75 | Indonezijščina Afrikaans švedščina |
poljščina
- baskovščina
- Katalonski
- Physicochemical parameters
Hindijščina
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Šona
Bolgarščina
- Cebuansko
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- hrvaščina
Nizozemščina
| Application object | Urdujščina Vietnamščina | Content in full-value feed (mg/kg) | Efficacy |
| gudžaratščina | Haitijščina | Havščina | kinjaruandščina Hmong madžarščina |
| Piglets and fattening pigs | Igboščina | Javanski | Kannada Kmerščina Kurdščina |
| Kirgizijščina | Latinsko | ||
| Bird | 300～400 | 45～60 | Makedonščina Malajščina Malajalamščina |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
norveščina
- Paštunščina
- Appearance: brownish-yellow granules
- Physicochemical parameters
Srbščina
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Šona
Sindhi
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
svahili
Tadžik
Tamilščina
Telugu
Tajska
| Application object | Urdujščina Vietnamščina | Content in full-value feed (mg/kg) | Efficacy |
| Jidiš | Jorubščina | Zulujščina | kinjaruandščina Oriščina Turkmenščina |
| Ujgurščina | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1,52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
